ACE Version 8.1+ will import any CAD/EDA file formats (like GDS-II, Gerber, ODB++, DXF), and seamlessly convert to a usable G-Code. G-code is the most widely used computer numerical control (CNC) programming language and used by most 3D Printers.
ACE can incorporate almost any external command-line G-Code generator (Slic3r, Prusa, CuraEngine, etc) which are very mature and have the ability to customize the G-Code output. The tutorial movie above used Slic3r command-line which can be directly downloaded/installed from their website, https://dl.slic3r.org/win/
Available Slic3r command line options, https://manual.slic3r.org/advanced/command-line